A simple method for analyzing actives in random RNAi screens: introducing the "H Score" for hit nomination & gene prioritization.

ABSTRACT: Due to the numerous challenges in hit identification from random RNAi screening, we have examined current practices with a discovery of a variety of methodologies employed and published in many reports; majority of them, unfortunately, do not address the minimum associated criteria for hit nomination, as this could potentially have been the cause or may well be the explanation as to the lack of confirmation and follow up studies, currently facing the RNAi field. Overall, we find that these criteria or parameters are not well defined, in most cases arbitrary in nature, and hence rendering it extremely difficult to judge the quality of and confidence in nominated hits across published studies. For this purpose, we have developed a simple method to score actives independent of assay readout;

SUBMITTER: Bhinder B 

PROVIDER: S-EPMC3678258 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature