Exogenous modulation of intrinsic optic nerve neuroprotective activity.

ABSTRACT:

Background

To characterize the molecular and functional status of the rat retina and optic nerve after acute elevation of intraocular pressure (IOP).

Methods

Retinal ischemia was induced in rats by increasing the IOP (110 mmHg/60 minutes). Microarray analysis, quantitative RT-PCR (qRT-PCR) and immunohistochemistry were used to characterize retinal tissue. PLGA microspheres containing neurotrophic factors (BDNF, GDNF, or CNTF) or empty microspheres were injected into the vitreous of operated animals 1 day after elevation of IOP. Pupil light reflex (PLR) parameters and electroretinograms (ERG) were monitored at multiple time points during the 60-day postoperative recovery period.

Results

Molecular analysis showed a significant intrinsic up-regulation of CNTF at 10 and 2

SUBMITTER: Grozdanic SD 

PROVIDER: S-EPMC3678383 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature