Characterization of adherent bacteroidales from intestinal biopsies of children and young adults with inflammatory bowel disease.

ABSTRACT: There is extensive evidence implicating the intestinal microbiota in inflammatory bowel disease [IBD], but no microbial agent has been identified as a sole causative agent. Bacteroidales are numerically dominant intestinal organisms that associate with the mucosal surface and have properties that both positively and negatively affect the host. To determine precise numbers and species of Bacteroidales adherent to the mucosal surface in IBD patients, we performed a comprehensive culture based analysis of intestinal biopsies from pediatric Crohn's disease [CD], ulcerative colitis [UC], and control subjects. We obtained biopsies from 94 patients and used multiplex PCR or 16S rDNA sequencing of Bacteroidales isolates for species identification. Eighteen different Bacteroidales species were iden

SUBMITTER: Zitomersky NL 

PROVIDER: S-EPMC3679120 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature