A multidimensional approach to an in-depth proteomics analysis of transcriptional regulators in neuroblastoma cells.

ABSTRACT: The dynamic regulation of transcriptional events is fundamental to many aspects of neuronal cell functions. However, proteomics methods have not been routinely used in global neuroproteomics analyses of transcriptional regulators because they are much less abundant than the "house-keeping" proteins in cells and tissues. Recent improvements in both biochemical preparations of nuclear proteins and detection sensitivities of proteomics technologies have made the global analysis of nuclear transcriptional regulators possible. We report here an optimised neuroproteomic method for the analysis of transcriptional regulators in the nuclear extracts of SHSY-5Y neuroblastoma cells by combining an improved nuclear protein extraction procedure with multidimensional peptide separation approaches. We fo

SUBMITTER: Li Q 

PROVIDER: S-EPMC3679315 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature