An endogenous artificial microRNA system for unraveling the function of root endosymbioses related genes in Medicago truncatula.

ABSTRACT:

Background

Legumes have the unique capacity to undergo two important root endosymbioses: the root nodule symbiosis and the arbuscular mycorrhizal symbiosis. Medicago truncatula is widely used to unravel the functions of genes during these root symbioses. Here we describe the development of an artificial microRNA (amiR)-mediated gene silencing system for M. truncatula roots.

Results

The endogenous microRNA (miR) mtr-miR159b was selected as a backbone molecule for driving amiR expression. Heterologous expression of mtr-miR159b-amiR constructs in tobacco showed that the backbone is functional and mediates an efficient gene silencing. amiR-mediated silencing of a visible marker was also effective after root transformation of M. truncatula constitutively expressing the visible ma

SUBMITTER: Devers EA 

PROVIDER: S-EPMC3679836 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature