Tet-mediated covalent labelling of 5-methylcytosine for its genome-wide detection and sequencing.

ABSTRACT: 5-methylcytosine is an epigenetic mark that affects a broad range of biological functions in mammals. The chemically inert methyl group prevents direct labelling for subsequent affinity purification and detection. Therefore, most current approaches for the analysis of 5-methylcytosine still have limitations of being either density-biased, lacking in robustness and consistency, or incapable of analysing 5-methylcytosine specifically. Here we present an approach, TAmC-Seq, which selectively tags 5-methylcytosine with an azide functionality that can be further labelled with a biotin for affinity purification, detection and genome-wide mapping. Using this covalent labelling approach, we demonstrate high sensitivity and specificity for known methylated loci, as well as increased CpG dinucleotid

SUBMITTER: Zhang L 

PROVIDER: S-EPMC3679896 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature