Production of L-lactic acid by the yeast Candida sonorensis expressing heterologous bacterial and fungal lactate dehydrogenases.

ABSTRACT:

Background

Polylactic acid is a renewable raw material that is increasingly used in the manufacture of bioplastics, which offers a more sustainable alternative to materials derived from fossil resources. Both lactic acid bacteria and genetically engineered yeast have been implemented in commercial scale in biotechnological production of lactic acid. In the present work, genes encoding L-lactate dehydrogenase (LDH) of Lactobacillus helveticus, Bacillus megaterium and Rhizopus oryzae were expressed in a new host organism, the non-conventional yeast Candida sonorensis, with or without the competing ethanol fermentation pathway.

Results

Each LDH strain produced substantial amounts of lactate, but the properties of the heterologous LDH affected the distribution of carbon between

SUBMITTER: Ilmen M 

PROVIDER: S-EPMC3680033 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature