Mechanistic study of a diazo dye degradation by Soybean Peroxidase.

ABSTRACT:

Background

Enzyme based remediation of wastewater is emerging as a novel, efficient and environmentally-friendlier approach. However, studies showing detailed mechanisms of enzyme mediated degradation of organic pollutants are not widely published.

Results

The present report describes a detailed study on the use of Soybean Peroxidase to efficiently degrade Trypan Blue, a diazo dye. In addition to examining various parameters that can affect the dye degradation ability of the enzyme, such as enzyme and H2O2 concentration, reaction pH and temperature, we carried out a detailed mechanistic study of Trypan Blue degradation. HPLC-DAD and LC-MS/MS studies were carried out to confirm dye degradation and analyze the intermediate metabolites and develop a detailed mechanistic dye deg

SUBMITTER: Kalsoom U 

PROVIDER: S-EPMC3680093 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature