A novel live-dead staining methodology to study malaria parasite viability.

ABSTRACT:

Background

Malaria is a major health and socio-economical problem in tropical and sub-tropical areas of the world. Several methodologies have been used to assess parasite viability during the adaption of field strains to culture or the assessment of drug potential, but these are in general not able to provide an accurate real-time assessment of whether parasites are alive or dead.

Methods

Different commercial dyes and kits were assessed for their potential to allow for the real-time detection of whether a blood stage malaria parasite is dead or alive.

Results

Here, a methodology is presented based on the potential-sensitive mitochondrial probe JC-1, which allows for the real-time visualization of live (red staining) and/or dead (absence of red staining) blood stage pa

SUBMITTER: Pasini EM 

PROVIDER: S-EPMC3680332 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature