A non-canonical mode of microtubule organization operates throughout pre-implantation development in mouse.

ABSTRACT: In dividing animal cells, the centrosome, comprising centrioles and surrounding pericentriolar-material (PCM), is the major interphase microtubule-organizing center (MTOC), arranging a polarized array of microtubules (MTs) that controls cellular architecture. The mouse embryo is a unique setting for investigating the role of centrosomes in MT organization, since the early embryo is acentrosomal, and centrosomes emerge de novo during early cleavages. Here we use embryos from a GFP::CETN2 transgenic mouse to observe the emergence of centrosomes and centrioles in embryos, and show that unfocused acentriolar centrosomes first form in morulae (~16-32-cell stage) and become focused at the blastocyst stage (~64-128 cells) concomitant with the emergence of centrioles. We then used high-resolution

SUBMITTER: Howe K 

PROVIDER: S-EPMC3680541 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature