Towards better understanding of an industrial cell factory: investigating the feasibility of real-time metabolic flux analysis in Pichia pastoris.

ABSTRACT:

Background

Novel analytical tools, which shorten the long and costly development cycles of biopharmaceuticals are essential. Metabolic flux analysis (MFA) shows great promise in improving our understanding of the metabolism of cell factories in bioreactors, but currently only provides information post-process using conventional off-line methods. MFA combined with real time multianalyte process monitoring techniques provides a valuable platform technology allowing real time insights into metabolic responses of cell factories in bioreactors. This could have a major impact in the bioprocessing industry, ultimately improving product consistency, productivity and shortening development cycles.

Results

This is the first investigation using Near Infrared Spectroscopy (NIRS) in situ

SUBMITTER: Fazenda ML 

PROVIDER: S-EPMC3681591 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature