Characterization of a novel swollenin from Penicillium oxalicum in facilitating enzymatic saccharification of cellulose.

ABSTRACT:

Background

Plant expansins and fungal swollenin that can disrupt crystalline cellulose have great potential for applications in conversion of biomass. Recent studies have been mainly focused on Trichoderma reesei swollenin that show relatively low activity in the promotion of cellulosic hydrolysis. Our aim was to isolate a novel swollenin with greater disruptive activity, to establish an efficient way of producing recombinant swollenin, and to optimize the procedure using swollenin in facilitation of cellulosic hydrolysis.

Results

A novel gene encoding a swollenin-like protein, POSWOI, was isolated from the filamentous fungus Penicillium oxalicum by Thermal Asymmetric Interlaced PCR (TAIL-PCR). It consisted of a family 1 carbohydrate-binding module (CBM1) followed by a linke

SUBMITTER: Kang K 

PROVIDER: S-EPMC3681723 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature