Genetic inactivation of acrAB or inhibition of efflux induces expression of ramA.

ABSTRACT:

Objectives

The transcriptional activator RamA regulates production of the multidrug resistance efflux AcrAB-TolC system in several Enterobacteriaceae. This study investigated factors that lead to increased expression of ramA.

Methods

In order to monitor changes in ramA expression, the promoter region of ramA was fused to a gfp gene encoding an unstable green fluorescence protein (GFP) on the reporter plasmid, pMW82. The ramA reporter plasmid was transformed into Salmonella Typhimurium SL1344 and a ΔacrB mutant. The response of the reporter to subinhibitory concentrations of antibiotics, dyes, biocides, psychotropic agents and efflux inhibitors was measured during growth over a 5 h time period.

Results

Our data revealed that the expression of ramA was increased in a Δa

SUBMITTER: Lawler AJ 

PROVIDER: S-EPMC3682690 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature