Ultrasound-mediated vascular gene transfection by cavitation of endothelial-targeted cationic microbubbles.

ABSTRACT:

Objectives

Ultrasound-mediated gene delivery can be amplified by acoustic disruption of microbubble carriers that undergo cavitation. We hypothesized that endothelial targeting of microbubbles bearing cDNA is feasible and, through optimizing proximity to the vessel wall, increases the efficacy of gene transfection.

Background

Contrast ultrasound-mediated gene delivery is a promising approach for site-specific gene therapy, although there are concerns with the reproducibility of this technique and the safety when using high-power ultrasound.

Methods

Cationic lipid-shelled decafluorobutane microbubbles bearing a targeting moiety were prepared and compared with nontargeted microbubbles. Microbubble targeting efficiency to endothelial adhesion molecules (P-selectin or int

SUBMITTER: Xie A 

PROVIDER: S-EPMC3682923 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature