Splicing kinetics and transcript release from the chromatin compartment limit the rate of Lipid A-induced gene expression.

ABSTRACT: The expression of eukaryotic mRNAs is achieved though an intricate series of molecular processes that provide many steps for regulating the production of a final gene product. However, the relationships between individual steps in mRNA biosynthesis and the rates at which they occur are poorly understood. By applying RNA-seq to chromatin-associated and soluble nucleoplasmic fractions of RNA from Lipid A-stimulated macrophages, we examined the timing of exon ligation and transcript release from chromatin relative to the induction of transcription. We find that for a subset of genes in the Lipid A response, the ligation of certain exon pairs is delayed relative to the synthesis of the complete transcript. In contrast, 3' end cleavage and polyadenylation occur rapidly once transcription extend

SUBMITTER: Pandya-Jones A 

PROVIDER: S-EPMC3683915 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature