Squish and CuAAC: additive-free covalent monolayers of discrete molecules in seconds.

ABSTRACT: A terminal alkyne is immobilized rapidly into a full monolayer by squishing a small volume of a solution of the alkyne between an azide-modified surface and a copper plate. The monolayer is covalently attached to the surface through a copper-catalyzed alkyne-azide cycloaddition (CuAAC) reaction, and the coverages of the immobilized electroactive alkyne species are quantified by cyclic voltammetry. A reaction time of less than 20 s is possible with no other reagents required. The procedure is effective under aerobic conditions using either an aqueous or aprotic organic solution of the alkyne (1-100 mM).

SUBMITTER: Pellow MA 

PROVIDER: S-EPMC3683963 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature