Identification of Bacillus anthracis specific chromosomal sequences by suppressive subtractive hybridization.

ABSTRACT:

Background

Bacillus anthracis, Bacillus thuringiensis and Bacillus cereus are closely related members of the B. cereus-group of bacilli. Suppressive subtractive hybridization (SSH) was used to identify specific chromosomal sequences unique to B. anthracis.

Results

Two SSH libraries were generated. Genomic DNA from plasmid-cured B. anthracis was used as the tester DNA in both libraries, while genomic DNA from either B. cereus or B. thuringiensis served as the driver DNA. Progressive screening of the libraries by colony filter and Southern blot analyses identified 29 different clones that were specific for the B. anthracis chromosome relative not only to the respective driver DNAs, but also to seven other different strains of B. cereus and B. thuringiensis included in the proc

SUBMITTER: Dwyer KG 

PROVIDER: S-EPMC368432 | biostudies-literature | 2004 Feb

REPOSITORIES: biostudies-literature