Pyrosequencing assessment of prokaryotic and eukaryotic diversity in biofilm communities from a French river.

ABSTRACT: Despite the recent and significant increase in the study of aquatic microbial communities, little is known about the microbial diversity of complex ecosystems such as running waters. This study investigated the biodiversity of biofilm communities formed in a river with 454 Sequencing™. This river has the particularity of integrating both organic and microbiological pollution, as receiver of agricultural pollution in its upstream catchment area and urban pollution through discharges of the wastewater treatment plant of the town of Billom. Different regions of the small subunit (SSU) ribosomal RNA gene were targeted using nine pairs of primers, either universal or specific for bacteria, eukarya, or archaea. Our aim was to characterize the widest range of rDNA sequences using different sets o

SUBMITTER: Bricheux G 

PROVIDER: S-EPMC3684755 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature