A one step real-time RT-PCR assay for the quantitation of Wheat yellow mosaic virus (WYMV).

ABSTRACT:

Background

Wheat yellow mosaic virus (WYMV) is an important pathogen in China and other countries. It is the member of the genus Bymovirus and transmitted primarily by Polymyxa graminis. The incidence of wheat infections in endemic areas has risen in recent years. Prompt and dependable identification of WYMV is a critical component of response to suspect cases.

Methods

In this study, a one step real-time RT-PCR, followed by standard curve analysis for the detection and identification of WYMV, was developed. Two reference genes, 18s RNA and β-actin were selected in order to adjust the veracity of the real-time RT-PCR assay.

Results

We developed a one-step Taqman-based real-time quantitative RT-PCR (RT-qPCR) assay targeting the conserved region of the 879 bp long full-l

SUBMITTER: Liu W 

PROVIDER: S-EPMC3685539 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature