Robustness of gut microbiota of healthy adults in response to probiotic intervention revealed by high-throughput pyrosequencing.

ABSTRACT: Probiotics are live microorganisms that potentially confer beneficial outcomes to host by modulating gut microbiota in the intestine. The aim of this study was to comprehensively investigate effects of probiotics on human intestinal microbiota using 454 pyrosequencing of bacterial 16S ribosomal RNA genes with an improved quantitative accuracy for evaluation of the bacterial composition. We obtained 158 faecal samples from 18 healthy adult Japanese who were subjected to intervention with 6 commercially available probiotics containing either Bifidobacterium or Lactobacillus strains. We then analysed and compared bacterial composition of the faecal samples collected before, during, and after probiotic intervention by Operational taxonomic units (OTUs) and UniFrac distances. The results showed

SUBMITTER: Kim SW 

PROVIDER: S-EPMC3686430 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature