Comprehensive analysis of the rice RING E3 ligase family reveals their functional diversity in response to abiotic stress.

ABSTRACT: A large number of really interesting new gene (RING) E3 ligases contribute to the post-translational modification of target proteins during plant responses to environmental stresses. However, the physical interactome of RING E3 ligases in rice remains largely unknown. Here, we evaluated the expression patterns of 47 Oryza sativa RING finger protein (OsRFP) genes in response to abiotic stresses via semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and in silico analysis. Subsequently, molecular dissection of nine OsRFPs was performed by the examination of their E3 ubiquitin ligase activity, subcellular localization, and physical interaction with target proteins. Most of the OsRFPs examined possessed E3 ligase activity and showed diverse subcellular localization. Yea

SUBMITTER: Lim SD 

PROVIDER: S-EPMC3686435 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature