Evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays.

ABSTRACT:

Background

Human adenoviruses (HAdVs) are the second-leading cause of childhood gastroenteritis worldwide. This virus is commonly found in environmental waters and is very resistant to water disinfection and environmental stressors, especially UV light inactivation. Molecular techniques, such as PCR-based methods (Polymerase Chain Reaction), are commonly used to detect and identify viral contamination in water, although PCR alone does not allow the discrimination between infectious and non-infectious viral particles. A combination of cell culture and PCR has allowed detection of infectious viruses that grow slowly or fail to produce cytopathic effects (CPE) in cell culture. This study aimed to assess the integrity and viability of human adenovirus (HAdV) in environmental water and

SUBMITTER: Fongaro G 

PROVIDER: S-EPMC3686584 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature