Endoplasmic reticulum stress induces fibrogenic activity in hepatic stellate cells through autophagy.

ABSTRACT:

Background & aims

Metabolic stress during liver injury enhances autophagy and provokes stellate cell activation, with secretion of scar matrix. Conditions that augment protein synthesis increase demands on the endoplasmic reticulum (ER) folding capacity and trigger the unfolded protein response (UPR) to cope with resulting ER stress. Generation of reactive oxygen species (ROS) is a common feature of hepatic fibrogenesis, and crosstalk between oxidant stress and ER stress has been proposed. The aim of our study was to determine the impact of oxidant and ER stress on stellate cell activation.

Methods

Oxidant stress was induced in hepatic stellate cells using H2O2 in culture or by ethanol feeding in vivo, and the UPR was analyzed. Because the branch of the UPR mainly affected w

SUBMITTER: Hernandez-Gea V 

PROVIDER: S-EPMC3686909 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature