Microfluidic genome-wide profiling of intrinsic electrical properties in Saccharomyces cerevisiae.

ABSTRACT: Methods to analyze the intrinsic physical properties of cells - for example, size, density, rigidity, or electrical properties - are an active area of interest in the microfluidics community. Although the physical properties of cells are determined at a fundamental level by gene expression, the relationship between the two remains exceptionally complex and poorly characterized, limiting the adoption of intrinsic separation technologies. To improve our current understanding of how a cell's genotype maps to a measurable physical characteristic and quantitatively investigate the potential of using these characteristics as biomarkers, we have developed a novel screen that combines microfluidic cell sorting with high-throughput sequencing and the haploid yeast deletion library to identify genes

SUBMITTER: Vahey MD 

PROVIDER: S-EPMC3686985 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature