Members of the Sinorhizobium meliloti ChvI regulon identified by a DNA binding screen.

ABSTRACT:

Background

The Sinorhizobium meliloti ExoS/ChvI two component regulatory system is required for N2-fixing symbiosis and exopolysaccharide synthesis. Orthologous systems are present in other Alphaproteobacteria, and in many instances have been shown to be necessary for normal interactions with corresponding eukaryotic hosts. Only a few transcriptional regulation targets have been determined, and as a result there is limited understanding of the mechanisms that are controlled by the system.

Results

In an attempt to better define the members of the regulon, we have applied a simple in vitro electrophoretic screen for DNA fragments that are bound by the ChvI response regulator protein. Several putative transcriptional targets were identified and three were further examined by re

SUBMITTER: Belanger L 

PROVIDER: S-EPMC3687685 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature