Integrative "omics"-approach discovers dynamic and regulatory features of bacterial stress responses.

ABSTRACT: Bacteria constantly face stress conditions and therefore mount specific responses to ensure adaptation and survival. Stress responses were believed to be predominantly regulated at the transcriptional level. In the phototrophic bacterium Rhodobacter sphaeroides the response to singlet oxygen is initiated by alternative sigma factors. Further adaptive mechanisms include post-transcriptional and post-translational events, which have to be considered to gain a deeper understanding of how sophisticated regulation networks operate. To address this issue, we integrated three layers of regulation: (1) total mRNA levels at different time-points revealed dynamics of the transcriptome, (2) mRNAs in polysome fractions reported on translational regulation (translatome), and (3) SILAC-based mass spectr

SUBMITTER: Berghoff BA 

PROVIDER: S-EPMC3688512 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature