Integrated transcriptomic and epigenomic analysis of primary human lung epithelial cell differentiation.

ABSTRACT: Elucidation of the epigenetic basis for cell-type specific gene regulation is key to gaining a full understanding of how the distinct phenotypes of differentiated cells are achieved and maintained. Here we examined how epigenetic changes are integrated with transcriptional activation to determine cell phenotype during differentiation. We performed epigenomic profiling in conjunction with transcriptomic profiling using in vitro differentiation of human primary alveolar epithelial cells (AEC). This model recapitulates an in vivo process in which AEC transition from one differentiated cell type to another during regeneration following lung injury. Interrogation of histone marks over time revealed enrichment of specific transcription factor binding motifs within regions of changing chromatin s

SUBMITTER: Marconett CN 

PROVIDER: S-EPMC3688557 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature