Mass fingerprinting of the venom and transcriptome of venom gland of scorpion Centruroides tecomanus.

ABSTRACT: Centruroides tecomanus is a Mexican scorpion endemic of the State of Colima, that causes human fatalities. This communication describes a proteome analysis obtained from milked venom and a transcriptome analysis from a cDNA library constructed from two pairs of venom glands of this scorpion. High perfomance liquid chromatography separation of soluble venom produced 80 fractions, from which at least 104 individual components were identified by mass spectrometry analysis, showing to contain molecular masses from 259 to 44,392 Da. Most of these components are within the expected molecular masses for Na(+)- and K(+)-channel specific toxic peptides, supporting the clinical findings of intoxication, when humans are stung by this scorpion. From the cDNA library 162 clones were randomly chosen, fr

SUBMITTER: Valdez-Velazquez LL 

PROVIDER: S-EPMC3688770 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature