Multiplex Identification of Human Papillomavirus 16 DNA Integration Sites in Cervical Carcinomas.

ABSTRACT: Cervical cancer is caused by high-risk human papillomaviruses (HPV), in more than half of the worldwide cases by HPV16. Viral DNA integration into the host genome is a frequent mutation in cervical carcinogenesis. Because integration occurs into different genomic locations, it creates unique viral-cellular DNA junctions in every single case. This singularity complicates the precise identification of HPV integration sites enormously. We report here the development of a novel multiplex strategy for sequence determination of HPV16 DNA integration sites. It includes DNA fragmentation and adapter tagging, PCR enrichment of the HPV16 early region, Illumina next-generation sequencing, data processing, and validation of candidate integration sites by junction-PCR. This strategy was performed with

SUBMITTER: Xu B 

PROVIDER: S-EPMC3688939 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature