Computational cloning of drug target genes of a parasitic nematode, Oesophagostomum dentatum.

ABSTRACT:

Background

Gene identification and sequence determination are critical requirements for many biological, genomic, and bioinformatic studies. With the advent of next generation sequencing (NGS) technologies, such determinations are predominantly accomplished in silico for organisms for which the genome is known or for which there exists substantial gene sequence information. Without detailed genomic/gene information, in silico sequence determination is not straightforward, and full coding sequence determination typically involves time- and labor-intensive PCR-based amplification and cloning methods.

Results

An improved method was developed with which to determine full length gene coding sequences in silico using de novo assembly of RNA-Seq data. The scheme improves upon initi

SUBMITTER: Romine NM 

PROVIDER: S-EPMC3689052 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature