Recombinant AAV9-TLK1B administration ameliorates fractionated radiation-induced xerostomia.

ABSTRACT: Salivary glands are highly susceptible to radiation, and patients with head and neck cancer treated with radiotherapy invariably suffer from its distressing side effect, salivary hypofunction. The reduction in saliva disrupts oral functions, and significantly impairs oral health. Previously, we demonstrated that adenoviral-mediated expression of Tousled-like kinase 1B (TLK1B) in rat submandibular glands preserves salivary function after single-dose ionizing radiation. To achieve long-term transgene expression for protection of salivary gland function against fractionated radiation, this study examines the usefulness of recombinant adeno-associated viral vector for TLK1B delivery. Lactated Ringers or AAV2/9 with either TLK1B or GFP expression cassette were retroductally delivered to rat sub

SUBMITTER: Timiri Shanmugam PS 

PROVIDER: S-EPMC3689188 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature