Suppression of antifolate resistance by targeting the myosin Va trafficking pathway in melanoma.

ABSTRACT: Human melanoma is a significant clinical problem. As most melanoma patients relapse with lethal drug-resistant disease, understanding and preventing mechanism(s) of resistance is one of the highest priorities to improve melanoma therapy. Melanosomal sequestration and the cellular exportation of cytotoxic drugs have been proposed to be important melanoma-specific mechanisms that contribute to multidrug resistance in melanoma. Concretely, we found that treatment of melanoma with methotrexate (MTX) altered melanogenesis and accelerated the exportation of melanosomes; however, the cellular and molecular processes by which MTX is trapped into melanosomes and exported out of cells have not been elucidated. In this study, we identified myosin Va (MyoVa) as a possible mediator of these cellular pr

SUBMITTER: Fernandez-Perez MP 

PROVIDER: S-EPMC3689245 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature