Dataset Information
Characterization of the transporterB0AT3 (Slc6a17) in the rodent central nervous system.
Ontology highlight
ABSTRACT: Background
The vesicular B0AT3 transporter (SLC6A17), one of the members of the SLC6 family, is a transporter for neutral amino acids and is exclusively expressed in brain. Here we provide a comprehensive expression profile of B0AT3 in mouse brain using in situ hybridization and immunohistochemistry.Results
We confirmed previous expression data from rat brain and used a novel custom made antibody to obtain detailed co-labelling with several cell type specific markers. B0AT3 was highly expressed in both inhibitory and excitatory neurons. The B0AT3 expression was highly overlapping with those of vesicular glutamate transporter 2 (VGLUT2) and vesicular glutamate transporter 1 (VGLUT1). We also show here that Slc6a17mRNA is up-regulated in animals subjected to short term food deprivation as well as animals treated with the serotonin reuptake inhibitor fluoxetine and the dopamine/noradrenaline reuptake inhibitor bupropion.Conclusions
This suggests that the B0AT3 transporter have a role in regulation of monoaminergic as well as glutamatergic synapses.
SUBMITTER: Hagglund MG
PROVIDER: S-EPMC3689596 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Publications
Characterization of the transporterB0AT3 (Slc6a17) in the rodent central nervous system.
BMC neuroscience 20130514
<h4>Background</h4>The vesicular B0AT3 transporter (SLC6A17), one of the members of the SLC6 family, is a transporter for neutral amino acids and is exclusively expressed in brain. Here we provide a comprehensive expression profile of B0AT3 in mouse brain using in situ hybridization and immunohistochemistry.<h4>Results</h4>We confirmed previous expression data from rat brain and used a novel custom made antibody to obtain detailed co-labelling with several cell type specific markers. B0AT3 was h ...[more]
