Combined methylation mapping of 5mC and 5hmC during early embryonic stages in bovine.

ABSTRACT:

Background

It was recently established that changes in methylation during development are dynamic and involve both methylation and demethylation processes. Yet, which genomic sites are changing and what are the contributions of methylation (5mC) and hydroxymethylation (5hmC) to this epigenetic remodeling is still unknown. When studying early development, options for methylation profiling are limited by the unavailability of sufficient DNA material from these scarce samples and limitations are aggravated in non-model species due to the lack of technological platforms. We therefore sought to obtain a representation of differentially 5mC or 5hmC loci during bovine early embryo stages through the use of three complementary methods, based on selective methyl-sensitive restriction and en

SUBMITTER: de Montera B 

PROVIDER: S-EPMC3689598 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature