Cryopreservation Causes Genetic and Epigenetic Changes in Zebrafish Genital Ridges.

ABSTRACT: Cryopreservation is an important tool routinely employed in Assisted Reproduction Technologies (ARTs) and germplasm banking. For several years, the assessment of global DNA fragmentation seemed to be enough to ensure the integrity of genetic material. However, cryopreservation can produce molecular alterations in key genes and transcripts undetectable by traditional assays, such modifications could interfere with normal embryo development. We used zebrafish as a model to study the effect of cryopreservation on key transcripts and genes. We employed an optimized cryopreservation protocol for genital ridges (GRs) containing primordial germ cells (PGCs) considered one of the best cell sources for gene banking. Our results indicated that cryopreservation produced a decrease in most of the zebr

SUBMITTER: Riesco MF 

PROVIDER: S-EPMC3689738 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature