Metabolomic signatures in lipid-loaded HepaRGs reveal pathways involved in steatotic progression.

ABSTRACT:

Objectives

A spectrum of disorders including simple steatosis, nonalcoholic steatohepatitis, fibrosis, and cirrhosis is described by nonalcoholic fatty liver disease (NAFLD). With the increased prevalence of obesity, and consequently NAFLD, there is a need for novel therapeutics in this area. To facilitate this effort, a cellular model of hepatic steatosis was developed using HepaRG cells and the resulting biochemical alterations were determined.

Design and methods

Using global metabolomic profiling, by means of a novel metabolite extraction procedure, the metabolic profiles in response to the saturated fatty acid palmitate, and a mixture of saturated and unsaturated fatty acids, palmitate and oleate (1:2) were examined.

Results

We observed elevated levels of the bran

SUBMITTER: Brown MV 

PROVIDER: S-EPMC3689848 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature