Fat accumulation in the liver of obese rats is alleviated by soy protein isolate through β-catenin signaling.

ABSTRACT:

Objectives

To investigate the effects of soy protein isolate (SPI) on Wnt/β-catenin signaling in the liver of obese rats, as well as the roles of this pathway in regulating the hepatic fat accumulation.

Design and methods

Obese and lean Zucker rats were fed diets containing either casein or SPI as protein source for 17 weeks. Histology and biochemical analysis, real-time PCR, Western blot, immunostaining, short interfering RNA assay were performed for liver samples.

Results

Our study showed that fat content was significantly lowered in the liver of SPI-fed obese rats, accompanied by a reduction in hepatocellular vacuolation, compared to the casein-fed control. β-Catenin protein level in the liver of obese rats was downregulated compared to the lean group, indicating t

SUBMITTER: Zhou D 

PROVIDER: S-EPMC3690171 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature