Optimized protocol for expression and purification of membrane-bound PglB, a bacterial oligosaccharyl transferase.

ABSTRACT: Asparagine-linked glycosylation (NLG) plays a significant role in a diverse range of cellular processes, including protein signaling and trafficking, the immunologic response, and immune system evasion by pathogens. A major impediment to NLG-related research is an incomplete understanding of the central enzyme in the biosynthetic pathway, the oligosaccharyl transferase (OTase). Characterization of the OTase is critical for developing ways to inhibit, engineer, and otherwise manipulate the enzyme for research and therapeutic purposes. The minimal understanding of this enzyme can be attributed to its complex, transmembrane structure, and the resulting instability and resistance to overexpression and purification. The following article describes an optimized procedure for recombinant expressi

SUBMITTER: Jaffee MB 

PROVIDER: S-EPMC3690457 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature