Swi/Snf chromatin remodeling/tumor suppressor complex establishes nucleosome occupancy at target promoters.

ABSTRACT: Precise nucleosome-positioning patterns at promoters are thought to be crucial for faithful transcriptional regulation. However, the mechanisms by which these patterns are established, are dynamically maintained, and subsequently contribute to transcriptional control are poorly understood. The switch/sucrose non-fermentable chromatin remodeling complex, also known as the Brg1 associated factors complex, is a master developmental regulator and tumor suppressor capable of mobilizing nucleosomes in biochemical assays. However, its role in establishing the nucleosome landscape in vivo is unclear. Here we have inactivated Snf5 and Brg1, core subunits of the mammalian Swi/Snf complex, to evaluate their effects on chromatin structure and transcription levels genomewide. We find that inactivation

SUBMITTER: Tolstorukov MY 

PROVIDER: S-EPMC3690861 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature