The Aspergillus nidulans peripheral ER: disorganization by ER stress and persistence during mitosis.

ABSTRACT: The genetically amenable fungus Aspergillus nidulans is well suited for cell biology studies involving the secretory pathway and its relationship with hyphal tip growth by apical extension. We exploited live-cell epifluorescence microscopy of the ER labeled with the translocon component Sec63, endogenously tagged with GFP, to study the organization of 'secretory' ER domains. The Sec63 A. nidulans ER network includes brightly fluorescent peripheral strands and more faintly labeled nuclear envelopes. In hyphae, the most abundant peripheral ER structures correspond to plasma membrane-associated strands that are polarized, but do not invade the hyphal tip dome, at least in part because the subapical collar of endocytic actin patches constrict the cortical strands in this region. Thus the subap

SUBMITTER: Markina-Inarrairaegui A 

PROVIDER: S-EPMC3691152 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature