Molecular fingerprint of high fat diet induced urinary bladder metabolic dysfunction in a rat model.

ABSTRACT:

Aims/hypothesis

Diabetic voiding dysfunction has been reported in epidemiological dimension of individuals with diabetes mellitus. Animal models might provide new insights into the molecular mechanisms of this dysfunction to facilitate early diagnosis and to identify new drug targets for therapeutic interventions.

Methods

Thirty male Sprague-Dawley rats received either chow or high-fat diet for eleven weeks. Proteomic alterations were comparatively monitored in both groups to discover a molecular fingerprinting of the urinary bladder remodelling/dysfunction. Results were validated by ELISA, Western blotting and immunohistology.

Results

In the proteome analysis 383 proteins were identified and canonical pathway analysis revealed a significant up-regulation of acute pha

SUBMITTER: Oberbach A 

PROVIDER: S-EPMC3691244 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature