Different responses to mechanical injury in neonatal and adult ovine articular cartilage.

ABSTRACT:

Background

Articular cartilage injury remains a major challenge in orthopedic surgery. This study aimed to identify differences in gene expression and molecular responses between neonatal and adult articular cartilage during the healing of an injury.

Methods

An established in vitro model was used to compare the transcriptional response to cartilage injury in neonatal and adult sheep by microarray analysis of gene expression. Total RNA was isolated from tissue samples, linearly amplified, and 15,208 ovine probes were applied to cDNA microarray. Validation for selected genes was obtained by real-time quantitative polymerase chain reaction (RT-qPCR).

Results

We found 1,075 (11.6%) differentially expressed probe sets in adult injured cartilage relative to normal cartilage

SUBMITTER: Xue X 

PROVIDER: S-EPMC3691644 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature