Development of monoclonal antibody-based immunoassays for detection of Helicobacter pylori neutrophil-activating protein.

ABSTRACT: Neutrophil-activating protein (NAP) is a major virulence factor expressed by Helicobacter pylori isolates associated with severe chronic gastroduodenal inflammation and peptic ulcers. NAP is one of the main protective antigens and a target for vaccine development against Helicobacter infection. In addition, NAP is a potent immune stimulator with potential application as a general vaccine adjuvant and in treatment of allergic diseases and cancer immunotherapy. NAP-specific immunoassays are needed for both H. pylori diagnostics and characterization of NAP-based vaccines and immunomodulatory preparations. We generated a panel of NAP-specific monoclonal antibodies (MAbs) by immunization of BALB/c mice with synthetic NAP peptides. The antibody reactivity against recombinant or native NAP antige

SUBMITTER: Iankov ID 

PROVIDER: S-EPMC3691681 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature