QARIP: a web server for quantitative proteomic analysis of regulated intramembrane proteolysis.

ABSTRACT: Regulated intramembrane proteolysis (RIP) is a critical mechanism for intercellular communication and regulates the function of membrane proteins through sequential proteolysis. RIP typically starts with ectodomain shedding of membrane proteins by extracellular membrane-bound proteases followed by intramembrane proteolysis of the resulting membrane-tethered fragment. However, for the majority of RIP proteases the corresponding substrates and thus, their functions, remain unknown. Proteome-wide identification of RIP protease substrates is possible by mass spectrometry-based quantitative comparison of RIP substrates or their cleavage products between different biological states. However, this requires quantification of peptides from only the ectodomain or cytoplasmic domain. Current analysis

SUBMITTER: Ivankov DN 

PROVIDER: S-EPMC3692105 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature