Cell cycle requirements for transduction by foamy virus vectors compared to those of oncovirus and lentivirus vectors.

ABSTRACT: Retroviral vectors based on foamy viruses (FV) are efficient gene delivery vehicles for therapeutic and research applications. While previous studies have shown that FV vectors transduce quiescent cell cultures more efficiently than oncoviral vectors, their specific cell cycle requirements have not been determined. Here we compare the transduction frequencies of FV vectors with those of onco- and lentiviral vectors in nondividing and dividing normal human fibroblasts by several methods. FV vectors transduced serum-deprived fibroblast cultures more efficiently than oncoretroviral vectors and at rates comparable to those of lentiviral vectors. However, in these cultures FV vectors only transduced a subpopulation of proliferating cells, as determined by bromodeoxyuridine staining for DNA synt

SUBMITTER: Trobridge G 

PROVIDER: S-EPMC369213 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature