Allele frequencies for 40 autosomal SNP loci typed for US population samples using electrospray ionization mass spectrometry.

ABSTRACT:

Aim

To type a set of 194 US African American, Caucasian, and Hispanic samples (self-declared ancestry) for 40 autosomal single nucleotide polymorphism (SNP) markers intended for human identification purposes.

Methods

Genotyping was performed on an automated commercial electrospray ionization time-of-flight mass spectrometer, the PLEX-ID. The 40 SNP markers were amplified in eight unique 5plex PCRs, desalted, and resolved based on amplicon mass. For each of the three US sample groups statistical analyses were performed on the resulting genotypes.

Results

The assay was found to be robust and capable of genotyping the 40 SNP markers consuming approximately 4 nanograms of template per sample. The combined random match probabilities for the 40 SNP assay ranged from 10-16 t

SUBMITTER: Kiesler KM 

PROVIDER: S-EPMC3692330 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature