Proteomic analysis of energy metabolism and signal transduction in irradiated melanoma cells.

ABSTRACT:

Aim

To analyze proteomic and signal transduction alterations in irradiated melanoma cells.

Methods

We combined stable isotope labeling with amino acids in cell culture (SILAC) with highly sensitive shotgun tandem mass spectrometry (MS) to create an efficient approach for protein quantification. Protein-protein interaction was used to analyze relationships among proteins.

Results

Energy metabolism protein levels were significantly different in glycolysis and not significantly different in oxidative phosphorylation after irradiation. Conversely, tumor suppressor proteins related to cell growth and development were downregulated, and those related to cell death and cell cycle were upregulated in irradiated cells.

Conclusion

Our results indicate that irradiation in

SUBMITTER: Yan LB 

PROVIDER: S-EPMC3693007 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature