An in vitro model of Mycobacterium leprae induced granuloma formation.

ABSTRACT:

Background

Leprosy is a contagious and chronic systemic granulomatous disease caused by Mycobacterium leprae. In the pathogenesis of leprosy, granulomas play a key role, however, the mechanisms of the formation and maintenance of M. leprae granulomas are still not clearly understood.

Methods

To better understand the molecular physiology of M. leprae granulomas and the interaction between the bacilli and human host cells, we developed an in vitro model of human granulomas, which mimicked the in vivo granulomas of leprosy. Macrophages were differentiated from human monocytes, and infected with M. leprae, and then cultured with autologous human peripheral blood mononuclear cells (PBMCs).

Results

Robust granuloma-like aggregates were obtained only when the M. leprae infec

SUBMITTER: Wang H 

PROVIDER: S-EPMC3693892 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature