Germline Mutations in Mtap Cooperate with Myc to Accelerate Tumorigenesis in Mice.

ABSTRACT:

Objective

The gene encoding the methionine salvage pathway methylthioadenosine phosphorylase (MTAP) is a tumor suppressor gene that is frequently inactivated in a wide variety of human cancers. In this study, we have examined if heterozygosity for a null mutation in Mtap (Mtap(lacZ)) could accelerate tumorigenesis development in two different mouse cancer models, Eμ-myc transgenic and Pten(+/-) .

Methods

Mtap Eμ-myc and Mtap Pten mice were generated and tumor-free survival was monitored over time. Tumors were also examined for a variety of histological and protein markers. In addition, microarray analysis was performed on the livers of Mtap(lacZ/+) and Mtap (+/+) mice.

Results

Survival in both models was significantly decreased in Mtap(lacZ/+) compared to Mtap(+/+) mi

SUBMITTER: Kadariya Y 

PROVIDER: S-EPMC3694069 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature