Inflammatory cytokines promote mesenchymal transformation in embryonic and adult valve endothelial cells.

ABSTRACT:

Objective

Inflammatory activation of valve endothelium is an early phase of aortic valve disease pathogenesis, but subsequent mechanisms are poorly understood. Adult valve endothelial cells retain the developmental ability to undergo endothelial-to-mesenchymal transformation (EndMT), but a biological role has not been established. Here, we test whether and how inflammatory cytokines (tumor necrosis factor-α and interleukin-6) regulate EndMT in embryonic and adult valve endothelium.

Methods and results

Using in vitro 3-dimensional collagen gel culture assays with primary cells, we determined that interleukin-6 and tumor necrosis factor-α induce EndMT and cell invasion in dose-dependent manners. Inflammatory-EndMT occurred through an Akt/nuclear factor-κB-dependent pathway in

SUBMITTER: Mahler GJ 

PROVIDER: S-EPMC3694265 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature